Typical Solution Users can create new plate definition files or modify existing ones in the Plate Geometry Editor of SparkControl. 9 963 views 1 year ago Tecan Knowledge Portal This video tutorial provides a step-by-step guide on how to install your new Infinite 200 PRO and i-control software, from unboxing to your first. IntroductionThe BacTiter-Glo Microbial Cell Viability Assay is a luminescence-based assay for the determination of the viability of microbial cells, using quantification of ATP as an indicator of metabolically active, viable cells (1). Simply enter the serial number of the QC plate, put the plate into the microplate reader, and press start. Tecan ends support for GENios plate reader Tecan was founded in 1980 by four engineers in the Swiss village of Hombrechtikon, with the aim to develop measurement, analysis and laboratory automation devices. Thanks to its dual-channel injector module, the Infinite 200 PRO is one of the few DLReady readers on the market, meaning it is certified for Promegas Dual Luciferase Reporter assays. How to Install your Infinite F50 and Magellan software. ***************************************************************************************. document.write(new Date().getFullYear());, Tecan Trading AG, Switzerland, all rights reserved. According to the Lambert-Beer law, the amount of absorbed light is proportional to the concentration of the sample, and to the pathlength the light has to pass through when going through the sample. In healthy individuals, intracellular antioxidant systems maintain ROS levels below a critical threshold, permitting essential ROSmediated signaling processes to function, but preventing ROS overproduction and potential tissue damage [1]. Use Tecan Spark multimode microplate reader to measure absorbance or fluorescence intensity of green fluorescence protein. Getting started learning tutorials, virtual classrooms, example files and nine different GUI languages are available. Kinetic data analysis with calculation of slopes, onsets and enzyme kinetics; calculation of Michaelis-Menten constant according to Lineweaver-Burk, Eadie-Hofstee and Hanes equations. . *************************************************************************************** Supports all major curve fittings, including: QC functions, such as Levy-Jennings graphs for trending of standards and controls, Available in nine languages, including English, French, German, Italian, Spanish, Portuguese, Russian, Chinese and Japanese, Step-by-step guidance for measurement set-up, Round filters for installation into four-position filter slides, Rectangular filters for installation into six-position filter slides, Accuracy, linearity and precision of the optics (NIST traceable). Automated data transfer to Magellan software and data export in ASCII and ASTM formats. The Sunrise plate reader, together with the Magellan reader control and data analysis software, is an ideal solution for a range of applications, including ELISAs, enzyme kinetic studies and many more. The Quad4 Monochromators of the Infinite M200 PRO offer industry-leading 260 nm wavelength accuracy (typically <0.5 %) and precision (typically <0.2 %) and, together with the NanoQuant Plate, provide a typical dsDNA detection limit of 1 ng/l with an A260/280 ratio of over 1.8. *************************************************************************************** Product number. Page 2 T +44 118 930 0300 Toll Free US: helpdesk-sy@tecan.com F +44 118 930 5671 T +1 800 TECAN US or helpdesk-uk@tecan.com T +1 800 832 2687 helpdesk . This makes filter slide handling very convenient, as the system will automatically recognize the filters installed on each slide. i-control takes care of everything, correlating and displaying all of your results in one single data set. There are now six application-tailored configurations for the acclaimed and proven Infinite 200 PRO plate reader, including new options such as automated pathlength correction. Principle of FluoroBlok cell culture insertsBD Falcon FluoroBlok cell culture inserts allow easy and non-destructive analysis of invasion and migration assays, as well as offering automation compatibility (1). What does an overflow or an OVER result mean? By continuing to browse our website, you accept our cookie policy. *************************************************************************************** The Infinite 200 PRO provides accurate and highly reproducible absorbance measurement capabilities, over a spectral range of 230 to 1000 nm. *************************************************************************************** *************************************************************************************** In this application note we describe the use of Tecans HydroFlexTM washer equipped with a magnetic bead plate carrier for fast purification of a large number of samples using magnetic beads.For the detection of the luminescence signal Tecans Infinite F200 multimode reader was used. The Infinite F50 Plus and Infinite F50 Robotic are Tecan's family of reliable, validated and compact absorbance microplate readers for ELISA. Tecan - Microplate Reader - Infinite 200 Pro - DKSH Product - @dkshgroup The Spark multimode reader from Tecan performs accurate and sensitive measurements of fluorescent cell-based assays. DNA-Expert reader. Tecan is a leading global provider of automated . Adherent cell types are typically analyzed through the well bottom in order to bring the cell monolayer as close as possible to the detector and avoid unspecific fluorescence noise by the overlying growth medium. Service Support Support Four channels for support. 5.14 When unit is finished measurements, remove standard plate and place back in protective case. The most widely established technology for UV-Vis absorbance measurement is a monochromator-based microplate reader. It features an optical fiber optimized for up to 384-well plates, plus optional 384-well injection capabilities, delivering great sensitivity and a large dynamic signal range for your assays. The optional temperature control function is ideal for enzyme kinetic studies up to 42 C. Get more from your microplate reader with Magellan, the universal reader control and data analysis software from Tecan. The following key reader parameters are checked according to pass/fail criteria, *************************************************************************************** This note describes the use of the fluorescence microplate reader Tecan Infinite F200 PRO or M200 PRO, for detection of as lias 20 pg dsDNA in a 200 l assay volume. Tab 03 / Software and FI., Filter optics for Luminescence. ***************************************************************************************. The use of dedicated AlphaLISA optics permits the analysis of target molecules in blood and serum by drastically reducing the effect of hemoglobin within a sample.The new module for AlphaScreen and AlphaLISA assays is the latest addition to the Infinite F200 PROs multimode functionality. *************************************************************************************** This saves time and gives you consistent and reliable performance. Simply enter the serial number of the QC plate, put the plate into the microplate reader, and press start. This proven platform has now evolved into a budget-friendly reader family consisting of six application-focused configurations, delivering performance and value to every lab, every day, for every assay. The Transcreener ADP2 TR-FRET Red Assay is intended for the detection of ADP production by any kinase or ATPase, and has been developed specifically for high throughput screening applications. The luminescence module is also completely separate from the fluorescence and absorbance optics, ensuring superb performance for glow, flash and dual-color luminescence applications. ***************************************************************************************. The Infinite 200 PRO NanoQuant is the ideal solution for a broad range of applications for measuring DNA- or RNA- quantification, for quality control and labeling efficiency. Expression of the reporter protein can be accomplished by measuring the protein itself or the enzymatic activity of the protein. BCA). The new Infinite readers offer affordable high performance detection solutions, powered by monochromator- and filter based technologies. ***************************************************************************************. This application note describes the use of the Infinite F200 PRO in combination with a commercially available ORAC assay kit, using different beverages as antioxidant samples Fluorescence-based drug sensitivity testing using 3D tumor microtissues and the Infinite M200 PRO monochromator-based multimode reader. ** Better than x % of measurement value plus corresponding OD value.Note: All deviations from the measurement value are meant in positive and negative directions. All major curve fittings are provided point to point, linear regression, non-linear regression, polynomial, cubic spline, Akima, LogitLog, four- and five-parameter fit. Complex workflows made easy i-control allows you to create a workflow for each application, using 'drag and drop' processing steps . For convenience and optimal data quality, the NanoQuant Plate is the only low volume plate on the market that is 100 % calibration free. By continuing to browse our website, you accept our cookie policy. An entire 96-well plate can be processed in less than six seconds. 2 Instructions For Use for SUNRISE Absorbance Reader No. These QC packages enable functional checks of the key instrument parameters such as accuracy, precision and linearity as well as microplate carrier alignment. QC Pac 2 offers comprehensive function checks for a typical range up to 1.6 OD, and is therefore often used for the IQOQ of the Sunrise reader. Here we describe the preparation and evaluation of RNA samples from a novel population of mouse keratinocyte stem cells marked by Lgr5+ expression. The Infinite F200 PROs filter-based fluorescence optics represent an affordable solution for both basic assays and advanced screening applications, such as FP, HTRF and TR-FRET. In vitro cytotoxicity is largely influenced by test article concentration and the exposure period with cells. The NanoQuant Plate from Tecan allows researchers to measure the concentration and purity of nucleic acids of up to 16 samples simultaneously. Support methods Local contact By continuing to browse our website, you accept our cookie policy. The z-position dialog of the instrument menu of Infinite M200 PRO provides an additional function which allows performing z-position scans for both a signal and a blank well for each fluorescent top measurement label. Specifications are subject to change without notice. From the Instrument tab select . Magellan supports the complete line of Tecan microplate readers enabling quick and easy measurements of samples for both first-time and expert users alike. They have been reported to be involved in anumber of diseases associated with excessive cell proliferation including atherosclerosis and cancer and are therefore often targeted in drug development and highthroughput screening (HTS) approaches.AlphaScreen-based phosphotyrosine assay kits, for example P-Tyr-100, have been developed for reliable and sensitive detection of kinase activity. The Sunrise absorbance reader, complemented by Tecans Magellan universal reader control and data analysis software add-on, is an ideal solution for a range of applications, such as ELISAs, enzyme kinetics, cell viability studies, veterinary assays and many more. One of the most used methods for determining nucleic acid concentrations is based on measuring the optical density of a sample at 260 nm (OD260). IntroductionCell-based assays are powerful and versatile tools for experimental design in life science research, and are frequently used for high throughput screening analysis. Tab 01 / Overview *************************************************************************************** Mikroplatten-Reader - Tecan PDA-based readers incorporate an optical grating and a solid state array detector, enabling measurement of light intensity throughout the UV and visible regions of the spectrum. Adenosine diphosphate (ADP) is an important mediator of cellular metabolism that is essential for maintaining energy levels. Full qualitative and quantitative, ELISAs, endpoint assays analyses. Cells that fail to compensate and neutralize heightened ROS levels die by apoptosis to avoid passing on ROS-caused DNA damage to daughter cells. SelectScience Independent Product Reviews, 12 measurement channels, 1 reference channel, 400 to 700 nm using gradient filter/340 to 750 nm using standard filter. Clinical mass spectrometry sample preparation. Standard applications, such as fluorescence-based DNA/RNA quantification assays, can be performed in both microplates and Tecans low volume NanoQuant Plate. Compact. The attenuation filter allows the PMT to operate in its most sensitive mode while still providing maximum performance for both weak and strong signals. In addition, the Infinite F200 PRO is now capable of measuring AlphaScreen- and AlphaLISA-based assays.The Infinite F200 PRO uses its fluorescence module in combination with a dedicated dichroic mirror and optimized filter sets for AlphaScreen and AlphaLISA applications. The following wizards are available: Magellan Standard is the software version for research and offers powerful data reduction such as: Magellan Tracker offers the functionality of the Standard version and fulfills the regulatory requirements of the FDA regulation 21 CFR part 11: Magellan offers a wide selection of predefined plate formats. The MultiCheck Plus QC plate is designed as a universal QC plate for the Infinite 200 PRO and Spark families of multimode microplate readers. ***************************************************************************************. Microplate Readers | Chobanian & Avedisian School of Medicine This enables simultaneous incubation and signal detection without the need for any manual intervention, even for microorganisms which need very specific environmental conditions, such as facultative anaerobic bacteria.In this collaborative study, Tecan and its partners have analyzed the growth of the human pathogen and class-I carcinogen Helicobacter pylori over a period of 28 hours.As a microaerophilic organism, H. pylori needs low atmospheric oxygen concentrations for optimized growth, and normally colonizes human mucosa, where it can cause certaintypes of stomach disorders and cancer. The Infinite 200 PROs Te-Inject module comes with a 1 ml syringe, and can be equipped with one or two channels. The dynamics of the signal inhibition, expressed as the area under the curve (AUC), are used to quantify the antioxidant capacity, expressed as the ORAC value, by comparing the sample AUC to an antioxidant standard curve generated with Trolox, a water-soluble vitamin E analog.
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